Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diverse Bacterial Properties Influence Dispersal Along Fungal Networks.

Journal of fungi (Basel, Switzerland)·2026
Same author

CXCL13-CXCR5 Signaling in CD8<sup>+</sup> T Cell Recruitment and Lymphoid Immune Organization in Clear Cell Renal Cell Carcinoma.

bioRxiv : the preprint server for biology·2026
Same author

A Head and Neck Cancer Patient-Specific Microphysiological System for Predicting Response to Chemoradiation.

bioRxiv : the preprint server for biology·2026
Same author

Lymphatic Endothelial Cells Regulate Neutrophil Phenotypes and Function in a Microphysiological Model of Infection.

bioRxiv : the preprint server for biology·2026
Same author

Multiplexed luminal tissue constructs with reconfigurable barriers for dynamic modeling of multi-tissue interactions.

Advanced materials technologies·2026
Same author

Prostate Cancer-Associated Fibroblasts: A Review on CAF Functions, Heterogeneity, Resistance Mechanisms, and Future in a Chip.

International journal of molecular sciences·2026
Same journal

Kinship Inferences for Second-Degree Relatives With a Combination of STRs and Microhaplotypes.

Electrophoresis·2026
Same journal

Optimisation of Electrokinetic Extraction System: Colourimetric Determination of Copper (II) in Sand Using Polymer Inclusion Membrane.

Electrophoresis·2026
Same journal

Novel Phloroglucinol Derivatives as Neuraminidase Inhibitors Identified From Humulus lupulus L. Extract by At-Line Nanofractionation Platform.

Electrophoresis·2026
Same journal

Protein-Based High-Performance Liquid Chromatography and Cyclodextrin-Capillary Electrokinetic Chromatography for the Chiral Separation of Azoles.

Electrophoresis·2026
Same journal

Dynamics of Heparin Translocations Through Solid-State Nanopores.

Electrophoresis·2026
Same journal

Production of Protein Hydrolysates and Bioactive Peptides From Lablab purpureus and Macrotyloma uniflorum via Optimized Extraction and Proteolysis Protocols.

Electrophoresis·2026
See all related articles

Related Experiment Video

Updated: Jun 23, 2026

Capillary-based Centrifugal Microfluidic Device for Size-controllable Formation of Monodisperse Microdroplets
08:20

Capillary-based Centrifugal Microfluidic Device for Size-controllable Formation of Monodisperse Microdroplets

Published on: February 22, 2016

An accessible micro-capillary electrophoresis device using surface-tension-driven flow.

Swomitra K Mohanty1, Jay Warrick, Jack Gorski

  • 1Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA. swomitra@gmail.com

Electrophoresis
|May 9, 2009
PubMed
Summary
This summary is machine-generated.

We developed an inexpensive micro-capillary electrophoresis chip using passive pumping for DNA analysis. This rapid fabrication method eliminates complex equipment, making DNA electrophoresis more accessible for labs.

More Related Videos

Microtensiometer for Confocal Microscopy Visualization of Dynamic Interfaces
08:05

Microtensiometer for Confocal Microscopy Visualization of Dynamic Interfaces

Published on: September 9, 2022

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System
14:12

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Published on: November 21, 2023

Related Experiment Videos

Last Updated: Jun 23, 2026

Capillary-based Centrifugal Microfluidic Device for Size-controllable Formation of Monodisperse Microdroplets
08:20

Capillary-based Centrifugal Microfluidic Device for Size-controllable Formation of Monodisperse Microdroplets

Published on: February 22, 2016

Microtensiometer for Confocal Microscopy Visualization of Dynamic Interfaces
08:05

Microtensiometer for Confocal Microscopy Visualization of Dynamic Interfaces

Published on: September 9, 2022

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System
14:12

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Published on: November 21, 2023

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Microfluidics

Background:

  • Micro-capillary electrophoresis (MCE) chips typically require complex interfacing equipment.
  • Existing fabrication methods for MCE chips often necessitate specialized facilities like cleanrooms.
  • Passive pumping methods offer a simplified approach to fluid handling in microfluidic devices.

Purpose of the Study:

  • To present a rapidly fabricated micro-capillary electrophoresis chip.
  • To demonstrate the use of surface-tension-driven flow (passive pumping) for DNA sample injection and extraction.
  • To create an accessible and inexpensive MCE device for routine laboratory use.

Main Methods:

  • Fabrication of the MCE chip using liquid phase photopolymerization of poly-isobornyl acrylate and glass.
  • Utilizing surface-tension-driven flow for precise sample injection and band extraction, eliminating the need for external pumps or valves.
  • Employing a standard microscope with a UV source for DNA detection and hydroxyl ethyl cellulose (HEC) for channel coating to suppress electroosmotic flow.

Main Results:

  • Rapid batch fabrication of the MCE chip in 1.5 hours, including channel coating.
  • Successful demonstration of DNA separations using a 100 bp ladder and characterization of 91 and 118 bp oligonucleotides.
  • Elimination of the need for tubing, valves, and syringe pumps through passive pumping, simplifying device operation.

Conclusions:

  • The developed MCE chip offers a cost-effective and rapidly fabricated solution for DNA analysis.
  • Passive pumping significantly simplifies sample handling and device interfacing in microelectrophoresis.
  • The device's reliance on standard laboratory equipment enhances its accessibility for a wider range of users.