Related Experiment Video
Updated: Jun 23, 2026
Non-destructive SPE-UPLC-based Quantification of Aflatoxins and Stilbenoid Phytoalexins in Single Peanut (Arachis spp.) Seeds
Published on: April 19, 2024
Product-specific sample clean-up and HPLC analysis of aflatoxins for a dietary product
Michael Zahn1, Mijeong Lee Jeong, Desheng Wang
1Unigen Inc., 2660 Willamette Drive NE, Lacey, WA 98516, USA.
Introduction:
A sample clean-up procedure has been developed for a complex dietary ingredient, Unigen Inc. (Lacey, WA, USA) product A (a proprietary mixture of Scutellaria baicalensis and Acacia catechu extracts), for the determination of aflatoxins by HPLC.
Objective:
To develop an appropriate sample specific clean-up procedure that removes interferences from the sample for the determination of total aflatoxins B(1), B(2), G(1) and G(2) at or lower than 20 ppb by HPLC.
Methodology:
The sample specific clean-up procedure was developed based on the modification of AOAC method 990.33.
Results:
Coextract interferences were removed with the clean-up procedure. The recovery of total aflatoxins was 70% with RSD about 2%; the LOD and LOQ were 1.6 and 5.2 ppb, respectively, for total aflatoxins; the linearity R(2) > 0.99. None of the samples tested showed the presence of detectable aflatoxins.
Conclusion:
The modified clean-up method was effective to remove coextract interferences from Unigen product A samples.
