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Published on: October 30, 2013
Immunohistochemical detection of advanced glycation end products in human bladder with specific monoclonal antibody
Kenshi Matsumoto1, Yukio Fujiwara, Ryoji Nagai
1Department of Urology, Kumamoto University Graduate School of Medicine, Kumamoto, Japan. kenshilund@hotmail.com
Objective:
To investigate the accumulation of advanced glycation end products (AGE) in human bladder.
Methods:
Human bladder specimens were obtained from nine patients during radical cystectomy. Frozen sections were immunohistochemically analyzed by three different monoclonal anti-AGE antibodies such as anti-N(epsilon)-(carboxymethyl)lysine (CML), anti-imidazolone and anti-pentosidine antibodies. Bladder sections were stained with these antibodies by indirect immunoperoxidase methods. Double immunohistochemical staining with one of the anti-AGE antibodies or an anti-human macrophage antibody was also carried out.
Results:
We demonstrated that CML and pentosidine were accumulated in human bladder extracellularly as well as intracellularly, whereas any accumulation of imidazolone was not observed. Double immunohistochemical staining indicated that AGE-accumulated cells in human bladder were derived from macrophages.
Conclusions:
The present study demonstrated that AGE-structures such as CML and pentosidine are accumulated extracellularly in human bladder, and were endocytosed by tissue macrophages.
