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Arachidonic acid induced increase in intracellular free calcium in guinea-pig hepatocytes
Y Hirano1, S Abe, T Sawanobori
1Department of Cardiovascular Diseases, Tokyo Medical and Dental University, Japan.
The Japanese Journal of Physiology
|January 1, 1991
Abstract:
Arachidonic acid (AA) caused elevations of intracellular free Ca2+ concentration ([Ca2+]i) measured by fura-2 fluorescence digital imaging microscopy in isolated guinea-pig hepatocytes. AA-induced increase in [Ca2+]i could still be observed when extracellular Ca2+ was chelated by EGTA, and was not influenced by either cyclooxygenase or lipoxygenase inhibitors. These results suggest that AA itself rather than its metabolites has direct effects on intracellular Ca2+ mobilization, causing the elevation of [Ca2+]i.