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Single Molecule Methods for Monitoring Changes in Bilayer Elastic Properties
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Limitations due to unstirred layers in measuring channel response of excised membrane patch using rapid solution

D Y Qin1, A Yoshida, A Noma

  • 1Department of Physiology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.

The Japanese Journal of Physiology
|January 1, 1991
PubMed

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Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...