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Updated: Aug 11, 2026
Measuring Fast Calcium Fluxes in Cardiomyocytes
Published on: November 29, 2011
A microfluorometric method for simultaneous measurement of changes in cytosolic free calcium concentration and pH in
I Shibuya1, K Matsuyama, K Tanaka
1Department of Physiology, Yamagata University School of Medicine, Japan.
Abstract:
We describe a new system to measure changes in cytosolic free Ca concentration ([Ca2+]i) and pH (pHi) simultaneously using the dyes fura-2 and BCECF, and a random access camera with computer-operated synchronously-changing excitation and emission filters. Fluorescence intensities for fura-2 and BCECF were recorded from rat cardiac myocytes and calibrated in vivo using multiple-point determinations. [Ca2+]i and pHi were 88.6 +/- 32.8 nM and 7.16 +/- 0.12, respectively.
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