Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 23, 2026

Analyzing Mitochondrial Function in a Drosophila melanogaster PINK1B9-Null Mutant Using High-resolution Respirometry
09:20

Analyzing Mitochondrial Function in a Drosophila melanogaster PINK1B9-Null Mutant Using High-resolution Respirometry

Published on: November 10, 2023

Using functional genomics to study PINK1 and metabolic physiology.

Camilla Scheele1, Ola Larsson, James A Timmons

  • 1The Centre of Inflammation and Metabolism, Department of Infectious Diseases and CMRC, Rigshospitalet, The Faculty of Health Sciences, University of Copenhagen, Denmark.

Methods in Enzymology
|May 12, 2009
PubMed
Summary

Functional genomics uses high-throughput data to study gene expression but requires careful experimental design and physiological context for meaningful results. Researchers must consider concentration-dependent specificity, similar to pharmacology, when using gene knockdown technologies like small interfering RNA (siRNA).

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Elongationless start-stop elements are stress-resilient translation gates that are more repressive than uTranslons.

Nucleic acids research·2026
Same author

Imaging-Derived Sarcopenic Obesity and Cardiovascular Outcomes: Insights Into Heart Failure Risk and Muscle Biology.

Journal of the American College of Cardiology·2026
Same author

Mitochondrial ETF insufficiency drives neoplastic growth by selectively optimizing cancer bioenergetics.

eLife·2026
Same author

Comparison of CX-4945 and SGC-CK2-1 as inhibitors of CSNK2 using quantitative phosphoproteomics: Triple SILAC in combination with inhibitor-resistant CSNK2.

Current research in chemical biology·2026
Same author

A network-based atlas of human skeletal muscle aging.

medRxiv : the preprint server for health sciences·2026
Same author

Decoding the Integrated Stress Response of Pancreatic Cancer: Identifying a Serine-dependent Tumor Subset Under Metabolic Relationships With CAFs.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Area of Science:

  • Genomics
  • Molecular Biology
  • Pharmacology

Background:

  • Genome sequencing and high-throughput technologies like microarrays and quantitative polymerase chain reaction generate vast amounts of biological data.
  • Functional genomics aims to understand gene function and expression coordination in complex physiological states.
  • The interpretation of genomic data relies heavily on physiological context and rigorous experimental control.

Purpose of the Study:

  • To provide considerations and observations from functional genomic studies, particularly in the post-microarray analysis phase.
  • To highlight the importance of experimental control and physiological context in interpreting functional genomics data.
  • To discuss the application and caveats of gene knockdown technologies, such as small interfering RNA (siRNA), inspired by RNA interference (RNAi).

More Related Videos

Measurement of Oxygen Consumption Rate in Acute Striatal Slices from Adult Mice
07:41

Measurement of Oxygen Consumption Rate in Acute Striatal Slices from Adult Mice

Published on: June 8, 2022

Analyzing Oxygen Consumption Rate in Primary Cultured Mouse Neonatal Cardiomyocytes Using an Extracellular Flux Analyzer
11:26

Analyzing Oxygen Consumption Rate in Primary Cultured Mouse Neonatal Cardiomyocytes Using an Extracellular Flux Analyzer

Published on: February 13, 2019

Related Experiment Videos

Last Updated: Jun 23, 2026

Analyzing Mitochondrial Function in a Drosophila melanogaster PINK1B9-Null Mutant Using High-resolution Respirometry
09:20

Analyzing Mitochondrial Function in a Drosophila melanogaster PINK1B9-Null Mutant Using High-resolution Respirometry

Published on: November 10, 2023

Measurement of Oxygen Consumption Rate in Acute Striatal Slices from Adult Mice
07:41

Measurement of Oxygen Consumption Rate in Acute Striatal Slices from Adult Mice

Published on: June 8, 2022

Analyzing Oxygen Consumption Rate in Primary Cultured Mouse Neonatal Cardiomyocytes Using an Extracellular Flux Analyzer
11:26

Analyzing Oxygen Consumption Rate in Primary Cultured Mouse Neonatal Cardiomyocytes Using an Extracellular Flux Analyzer

Published on: February 13, 2019

Main Methods:

  • Application of functional genomics techniques following microarray analysis.
  • Design of chemical tools for gene transcript knockdown, including distinguishing splice variants.
  • Utilizing principles from pharmacology regarding concentration-dependent specificity in experimental design.

Main Results:

  • High-throughput genomic data can yield novel insights into gene expression but may be meaningless without proper context.
  • Gene knockdown technologies, while powerful, require careful consideration of specificity, analogous to pharmacological inhibitor studies.
  • Functional genomics studies in simplified cell biology systems can provide valuable insights but must be cautiously extrapolated.

Conclusions:

  • Functional genomics holds great promise for understanding metabolic physiology but requires meticulous attention to experimental design and context.
  • The application of gene silencing technologies necessitates an understanding of concentration-dependent effects to ensure specificity and reliable results.
  • Integrating insights from pharmacology and rigorous controls is crucial for the successful application of functional genomics in biological research.