Related Experiment Video
Updated: Jun 23, 2026
A Fluorescence Microscopy Assay for Monitoring Mitophagy in the Yeast Saccharomyces cerevisiae
Published on: July 18, 2011
Determination of yeast mitochondrial KHE activity, osmotic swelling and mitophagy
Karin Nowikovsky1, Rodney J Devenish, Elisabeth Froschauer
1Max F. Perutz Laboratories, Department of Genetics, University of Vienna, Campus Vienna Biocenter, Wien, Austria.
Abstract:
The mitochondrial K(+)/H(+) exchanger (KHE) is a key regulator of mitochondrial K(+), the most abundant cellular cation, and thus for volume control of the organelle. Downregulation of the mitochondrial KHE results in osmotic swelling and autophagic degradation of the organelle. This chapter describes methods to shut-off expression of Mdm38p, an essential factor of the mitochondrial KHE, and to observe the cellular consequences thereof, in particular changes in KHE activity and morphogenetic changes of mitochondria by applying new techniques developed in our laboratories.
