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A long-term conservation tool for cell characteristics: cryopreservation of Paramecium caudatum

Sascha Krenek1, Thomas U Berendonk

  • 1Molecular Evolution and Animal Systematics, Institute of Biology II, University of Leipzig, 04103 Leipzig, Germany. krenek@rz.uni-leipzig.de

Protist
|May 12, 2009
PubMed
Abstract

No abstract available in PubMed .

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...