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Updated: Jun 23, 2026

Lipid Exchange Assay in Living Cells
Published on: March 21, 2025
Glycine transporter 1 associates with cholesterol-rich membrane raft microdomains
Xin Liu1, Ann D Mitrovic, Robert J Vandenberg
1Transporter Biology Group, School of Medical Sciences (Pharmacology), Bosch Institute, University of Sydney, Sydney, NSW 2006, Australia.
Abstract:
Membrane rafts, the highly-ordered, cholesterol-rich microdomains of the plasma membrane play important roles in cellular functions. In this study, GLYT1-CFP and GLYT2-CFP were constructed, followed by investigation of whether the tagged transporters associate with a fluorescence probe that labels membrane rafts (DilC16) by using Fluorescence Resonance Energy Transfer. A close association was observed between DiIC16 and GLYT1-CFP, but not for GLYT2-CFP. The glycine transport ability of GLYT1 is also highly dependent on the integrity of this area. Together, the results suggest that GLYT1 and membrane rafts are co-localized in the membrane, and that this influences the rate of glycine transport.
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