Characterisation of a carp cell line for analysis of apoptosis

Teleost fish in general, and common carp in particular, are excellent genetic models for bridging the gap in knowledge between invertebrate models such as C. elegans and D. melanogaster, on one hand, and higher vertebrates on the other hand, although, until now, there have been few well characterised fish cell lines shown to be suitable for studies on apoptosis. The present study describes the suitability of a permanent, nonleukemic, nonvirally infected carp cell line for apoptotic studies. A traditional approach using known apoptotic inducers such as UV-light combined with RNA interference, the latest ready-to-use technology widely used in higher vertebrates, was tested in the carp leucocyte cell line (CLC). This study was designed as a first step towards a better knowledge of fish macrophages and their fate after different types of apoptotic insults.