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Barley-derived beta-D-glucan induces immunostimulation via a dectin-1-mediated pathway
Rui Tada1, Futoshi Ikeda, Kosuke Aoki
1Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1, Horinouchi, Hachioji, Tokyo, Japan.
Immunology Letters
|May 12, 2009
Summary
Barley beta-glucan activates the immune system by signaling through dectin-1. This pathway leads to NF-kappaB activation and cytokine production, suggesting a key role for dectin-1 in barley
Area of Science:
- Immunology
- Molecular Biology
- Biochemistry
Background:
- Barley beta-glucan is known to bind dectin-1.
- The signaling pathway activated by this interaction is not fully understood.
Purpose of the Study:
- To investigate if barley beta-glucan can trigger signal transduction via dectin-1.
- To determine the role of dectin-1 in mediating the immune effects of barley beta-glucan.
Main Methods:
- Reporter gene assay in 293T cells to assess NF-kappaB activation.
- Co-transfection of dectin-1, Syk, CARD9, and Bcl10.
- Analysis of Syk phosphorylation and IL-6 production in macrophages.
Main Results:
- Barley beta-glucan activated NF-kappaB and cytokine production when dectin-1, Syk, CARD9, and Bcl10 were coexpressed.
- Barley beta-glucan induced Syk phosphorylation and IL-6 production in macrophages.
Conclusions:
- Barley beta-glucan's immunostimulatory effects are, at least partly, mediated through dectin-1.
- The dectin-1, Syk, CARD9, and Bcl10 pathway is involved in barley beta-glucan's immune response.
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