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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.

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Related Experiment Video

Updated: Jun 23, 2026

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms
15:27

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

Published on: April 17, 2017

Antibody production, design and use for biosensor-based applications.

Paul J Conroy1, Stephen Hearty, Paul Leonard

  • 1Biomedical Diagnostics Institute, National Centre for Sensor Research, School of Biotechnology, Dublin City University, Dublin 9, Ireland.

Seminars in Cell & Developmental Biology
|May 12, 2009
PubMed
Summary

Antibody-based biosensors offer precise detection for diagnostics and contaminant monitoring. Recombinant antibody technologies enhance biosensor design, stability, affinity, and specificity for improved molecular recognition.

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Area of Science:

  • Biomedical Engineering
  • Immunotechnology
  • Analytical Chemistry

Background:

  • Reliable detection and quantification of analytes are vital across diverse fields.
  • Biosensors have transformed diagnostics, contaminant detection, and disease marker identification.
  • Antibodies are key components in biosensor devices due to their specific antigen recognition.

Purpose of the Study:

  • To review current antibody-based biosensor platforms.
  • To examine molecular biological techniques for antibody generation and improvement.
  • To highlight the advantages of recombinant antibodies in biosensor development.

Main Methods:

  • Review of existing literature on antibody biosensor platforms.
  • Critical analysis of molecular biological methods for antibody engineering.
  • Evaluation of antibody attributes (design, stability, affinity, specificity) for biosensors.

Main Results:

  • Antibodies are crucial for molecular recognition in various biosensor applications.
  • Molecular biological techniques enable the generation of improved antibodies.
  • Recombinant antibodies offer enhanced design, stability, affinity, and specificity for biosensors.

Conclusions:

  • Antibody-based biosensors are essential tools for sensitive and specific analyte detection.
  • Recombinant antibody technology significantly advances biosensor capabilities.
  • Future biosensor development will benefit from engineered antibodies with superior performance characteristics.