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Application of LC/MS to proteomics studies: current status and future prospects
Guodong Chen1, Birendra N Pramanik
1Schering-Plough Research Institute, 2015 Galloping Hill Road, Kenilworth, NJ 07033, USA. guodong.chen@bms.com
Abstract:
Liquid chromatography/mass spectrometry (LC/MS) has become a powerful technology in proteomics studies in drug discovery, including target protein characterization and discovery of biomarkers. This review article will describe current LC/MS approaches in protein characterization, including a bottom-up method for protein identification and quantitative proteomics. We will discuss the investigation of protein post-translational modifications such as glycosylation (glycoproteomics) and phosphorylation (phosphoproteomics) using LC/MS. Future trends in LC/MS with respect to proteomics studies will also be illustrated.
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