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Updated: Jun 23, 2026

Determining Membrane Protein Topology Using Fluorescence Protease Protection (FPP)
Published on: April 20, 2015
TOPCONS: consensus prediction of membrane protein topology
Andreas Bernsel1, Håkan Viklund, Aron Hennerdal
1Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, SE-106 91, Sweden.
Abstract:
TOPCONS (http://topcons.net/) is a web server for consensus prediction of membrane protein topology. The underlying algorithm combines an arbitrary number of topology predictions into one consensus prediction and quantifies the reliability of the prediction based on the level of agreement between the underlying methods, both on the protein level and on the level of individual TM regions. Benchmarking the method shows that overall performance levels match the best available topology prediction methods, and for sequences with high reliability scores, performance is increased by approximately 10 percentage points. The web interface allows for constraining parts of the sequence to a known inside/outside location, and detailed results are displayed both graphically and in text format.
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