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Related Concept Videos

DNA as a Genetic Template02:05

DNA as a Genetic Template

Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
DNA as a Genetic Template02:05

DNA as a Genetic Template

Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
DNA Topoisomerases02:02

DNA Topoisomerases

Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
Topoisomerases are divided into two main types.  Type I...
The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
DNA Replication02:40

DNA Replication

DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication uses a large number of...

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Design and Synthesis of a Reconfigurable DNA Accordion Rack
07:44

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Published on: August 15, 2018

Writing DNA with GenoCAD.

Michael J Czar1, Yizhi Cai, Jean Peccoud

  • 1Virginia Bioinformatics Institute at Virginia Polytechnic and State University, Blacksburg, VA 24061, USA.

Nucleic Acids Research
|May 12, 2009
PubMed
Summary
This summary is machine-generated.

GenoCAD is a free web application that simplifies synthetic DNA sequence design. It enables users to create complex genetic constructs quickly using a graphical interface and extensive part libraries.

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Area of Science:

  • Synthetic Biology
  • Bioinformatics

Background:

  • Designing custom DNA sequences for applications like protein expression vectors and artificial gene networks requires specialized software.
  • Current methods can be complex and time-consuming, necessitating streamlined approaches.

Purpose of the Study:

  • To introduce GenoCAD, a free, web-based application designed to facilitate the design of synthetic DNA constructs.
  • To provide a user-friendly platform for creating complex genetic designs through a graphical interface.

Main Methods:

  • GenoCAD utilizes grammatical models to capture DNA sequence design strategies.
  • Users interact with a graphical interface, clicking icons for structural features and genetic parts.
  • The application automatically derives construct sequences from integrated libraries of genetic parts.

Main Results:

  • Complex genetic constructs, including protein expression vectors and gene networks, can be designed in minutes.
  • GenoCAD automatically generates the final DNA sequence based on user design choices.
  • Users can download sequences for synthesis or further analysis.

Conclusions:

  • GenoCAD offers an efficient and intuitive solution for designing synthetic DNA sequences.
  • The application supports customization through personal accounts, allowing users to manage their own part libraries and designs.