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Large cryptic internal sequence repeats in protein structures from Homo sapiens.

R Sarani1, N A Udayaprakash, R Subashini

  • 1Bioinformatics Centre, Indian Institute of Science, Bangalore 560 012, India.

Journal of Biosciences
|May 12, 2009
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Summary

Large cryptic repeats in amino acid sequences are conserved despite mutations. This study investigates if these conserved sequences also maintain similar three-dimensional structures, impacting protein stability and disease.

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Area of Science:

  • Molecular Biology
  • Structural Biology
  • Bioinformatics

Background:

  • Amino acid sequences typically mutate and diverge over time.
  • However, certain large, cryptic repeats show remarkable sequence conservation.
  • The reasons for this selective conservation at the sequence level remain unclear.

Purpose of the Study:

  • To investigate the structural conservation of large cryptic repeats within protein sequences.
  • To determine if sequence-level conservation correlates with three-dimensional structural similarity.
  • To understand the role of these repeats in protein stability, function, and disease.

Main Methods:

  • Comparative sequence analysis of protein families.
  • Structural analysis using homology modeling and structural alignment.
  • Investigation of evolutionary constraints on repeat regions.

Main Results:

  • Identified specific large cryptic repeats exhibiting significant sequence conservation.
  • Demonstrated that these conserved sequences often maintain similar three-dimensional structural folds.
  • Found evidence suggesting these repeats are critical for protein stability.

Conclusions:

  • Sequence conservation in large cryptic repeats is linked to structural conservation.
  • These structural features play a crucial role in maintaining protein integrity and function.
  • Understanding these repeats offers insights into protein evolution and disease mechanisms.