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Updated: Jun 23, 2026

DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering
Published on: November 9, 2017
Positive charged polymer as a probe for DNA determination by resonance light scattering
Juan Du1, Huzhi Zheng, Chengzhi Huang
1Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Beibei, Chongqing 400715, PR China.
Abstract:
Electrostatic interactions of calf thymus DNA (ct-DNA) with a positive charged polymer were investigated based on measurements of enhanced resonance light scattering (RLS) signals, and were proved by scanning electron microscopy (SEM). The optimal pH value was 7.0 in a phosphate-buffered saline solution (PBS) and the maximum RLS peak located at 344.8 nm. The RLS signal enhancement (DeltaI(RLS)) could be linearly correlated with the concentration of ct-DNA in the range of 0.0900 to 2.70 microg mL(-1), and the detection limit (S/N = 3) was 17.0 ng mL(-1). The binding ratio and the binding constant between DNA and polymer were also provided.
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