TU-tagging: cell type-specific RNA isolation from intact complex tissues

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Published on: December 8, 2021

Michael R Miller1, Kristin J Robinson, Michael D Cleary

  • 1Institute of Neuroscience, Howard Hughes Medical Institute, University of Oregon, Eugene, Oregon, USA.

Nature Methods
|May 12, 2009
PubMed

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RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...