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Updated: Jun 23, 2026

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy
Published on: June 27, 2014
Nonlinear voltage dependence of the light-driven proton pump current of bacteriorhodopsin
Abstract:
The light-driven proton pump current generated by bacteriorhodopsin reconstituted in asymmetric planar bilayer membranes was investigated. The current-voltage dependence was found to be nonlinear and can be approximated by an exponential at least below +50 mV. The current changed e-fold when the membrane potential was changed by 80 mV. The voltage dependence was analyzed in terms of a barrier model. This analysis revealed an effective displacement of 0.63 elementary charges across the membrane during the rate-limiting step. Comparison of this value with the results from flash-induced photovoltage signals suggests that one proton is pumped per cycle.
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