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Updated: Jun 23, 2026

HPLC Measurement of the DNA Oxidation Biomarker, 8-oxo-7,8-dihydro-2’-deoxyguanosine, in Cultured Cells and Animal Tissues
Published on: August 1, 2015
[8-oxo-2'-deoxyguanosine accumulation in DNA from "stationary phase aging" cultured cells]
Abstract:
The 8-oxo-dG/dG ratio in DNA of cultured transformed Chinese hamster cells was analyzed during their "stationary phase aging". Amount of 8-oxo-dG and dG in DNA hydrolyzate was evaluated by HPLC-EC. The cells grew and "aged" for 15 days. As expected, the 8-oxo-dG/dG ratio increased with cell "age". It did not change significantly from 4th to 8th day (6.26 x 10(-5) and 4.42 x 10(-5), correspondingly) and then abruptly increased to 15th day of "age" (22.40 x 10(-5)). The results are in accordance with the conception of cell proliferation restriction as the starting mechanism of ageing and the method can be used for evaluation of cell culture biological age when testing new compounds for their geroprotector or geropromoter activity.
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