Related Experiment Video

Updated: Jun 23, 2026

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells
06:54

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells

Published on: October 27, 2020

Involvement of G-patch domain containing 2 overexpression in breast carcinogenesis

Meng-Lay Lin1, Chikako Fukukawa, Jae-Hyun Park

  • 1Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, The University of Tokyo, Tokyo, Japan.

Cancer Science
|May 13, 2009
PubMed

Insights

A novel gene, G-patch domain containing 2 (GPATCH2), is overexpressed in most breast cancers and crucial for cancer cell growth. Targeting GPATCH2 offers a potential new therapeutic strategy for breast cancer patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genomics

Background:

  • Breast cancer is a leading cause of mortality in women worldwide.
  • Identifying novel molecular targets is crucial for developing effective breast cancer therapies.

Purpose of the Study:

  • To identify novel genes involved in breast cancer pathogenesis.
  • To investigate the role of G-patch domain containing 2 (GPATCH2) in breast cancer cell growth and explore its therapeutic potential.

Main Methods:

  • Genome-wide gene expression profiling of 81 breast tumors.
  • siRNA-mediated knockdown of GPATCH2 in MCF-7 and T47D breast cancer cells.
  • Analysis of GPATCH2 protein interaction with hPrp43, an RNA-dependent ATPase.
  • Northern blot analysis of GPATCH2 expression in normal human organs.

Main Results:

  • GPATCH2 was found to be overexpressed in the majority of breast tumors analyzed.
  • Suppression of GPATCH2 expression using siRNA led to significant growth inhibition of breast cancer cells.
  • GPATCH2 interacts with hPrp43, enhancing its ATPase activity and promoting mammalian cell growth.
  • GPATCH2 exhibits characteristics of a cancer/testis antigen.

Conclusions:

  • GPATCH2 plays an essential role in breast cancer cell proliferation.
  • The interaction between GPATCH2 and hPrp43 is important for cell growth.
  • Targeting GPATCH2 or its interaction with hPrp43 represents a promising therapeutic strategy for breast cancer.

More Related Videos

Phospholipid Mediator Induced Transformation in Three-Dimensional Cultures08:02

Phospholipid Mediator Induced Transformation in Three-Dimensional Cultures

Published on: July 27, 2022

Related Concept Videos

GPCRs Regulate Adenylyl Cylase Activity01:09

GPCRs Regulate Adenylyl Cylase Activity

Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of cells.
Two...
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Activation and Inactivation of G Proteins01:22

Activation and Inactivation of G Proteins

Heterotrimeric G proteins are guanine nucleotide-binding proteins. As the name suggests, heterotrimeric G proteins are composed of three subunits: alpha, beta, and gamma. They remain GDP-bound or GTP-bound inside the cells and switch between inactive/active states. The Gα subunit possesses the nucleotide-binding pocket that binds guanine nucleotides and switches between GDP or GTP-bound states. In contrast, the Gꞵ and Gγ subunits are always bound together with high affinity and are together...
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...