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DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

DNA Isolation

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Related Experiment Video

Updated: Jun 23, 2026

Genotyping of Plant and Animal Samples without Prior DNA Purification

Genotyping of Plant and Animal Samples without Prior DNA Purification

Published on: September 24, 2012

High-quality plant DNA extraction for PCR: an easy approach.

I Ahmed¹, M Islam, W Arshad

¹Allan Wilson Centre for Molecular Ecology and Evolution, Massey University, Palmerston North, New Zealand. I.Ahmed@massey.ac.nz

Journal of Applied Genetics

|May 13, 2009

Summary

This study presents a simple, cost-effective plant DNA extraction method yielding high-quality DNA suitable for polymerase chain reaction (PCR) applications. The procedure ensures reproducible PCR results from various plant tissues, even after long-term DNA storage.

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Area of Science:

Molecular Biology
Plant Science
Biotechnology

Background:

Reproducible polymerase chain reaction (PCR) results depend on template DNA quantity and quality.
Genomic studies and genetic transformations require reliable DNA extraction methods.
Existing plant DNA extraction protocols can be complex or costly.

Purpose of the Study:

To develop a simple, efficient, and cost-effective plant DNA extraction procedure.
To isolate high-quality plant DNA suitable for various PCR applications.
To validate the DNA extraction method for different plant species and storage conditions.

Main Methods:

Maceration of plant tissue in a DNA extraction buffer (100 mM Tris-HCl, 100 mM EDTA, 250 mM NaCl).
Cell lysis using 20% SDS followed by DNA extraction with phenol:chloroform:iso-amyl alcohol (25:24:1).
Purification using hydrated ether to remove polysaccharides and contaminants.

Main Results:

Average DNA yield of 20-30 µg/cm² from fresh tissues.
Absorbance ratios (A260/A280) of 1.5-1.8, indicating good DNA purity.
Successful PCR amplification using microsatellite, RAPD, and specific markers from various plant species (barley, oat, potato, tomato).
Amplification achieved with DNA stored for over 2 years, demonstrating long-term stability.

Conclusions:

The presented method provides high-quality plant DNA suitable for PCR.
The procedure is efficient, cost-effective, and easy to use for diverse plant samples.
The extracted DNA is reliable for genetic analysis and long-term storage applications.