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Related Experiment Video

Updated: Jun 23, 2026

Predicting Gene Silencing Through the Spatiotemporal Control of siRNA Release from Photo-responsive Polymeric Nanocarriers
11:53

Predicting Gene Silencing Through the Spatiotemporal Control of siRNA Release from Photo-responsive Polymeric Nanocarriers

Published on: July 21, 2017

Photo-assisted gene delivery using light-responsive catanionic vesicles.

Yu-Chuan Liu1, Anne-Laure M Le Ny, Judith Schmidt

  • 1Department of Chemical Engineering and Materials Science, University of Southern California, Los Angeles, California 90089, USA.

Langmuir : the ACS Journal of Surfaces and Colloids
|May 14, 2009
PubMed
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Novel photoresponsive catanionic vesicles enhance gene delivery by improving cellular uptake and enabling light-triggered DNA release. These light-sensitive gene vectors show increased transfection efficiency in cells.

Area of Science:

  • Biotechnology
  • Materials Science
  • Molecular Biology

Background:

  • Gene delivery vectors are crucial for therapeutic applications.
  • Current vectors face challenges in cellular uptake and controlled payload release.
  • Photoresponsive materials offer potential for spatiotemporal control in biological systems.

Purpose of the Study:

  • To develop and characterize novel photoresponsive catanionic vesicles for gene delivery.
  • To investigate the mechanism of enhanced cellular uptake and phototriggered DNA release.
  • To evaluate the efficiency of these vesicles in gene transfection.

Main Methods:

  • Spontaneous formation of catanionic vesicles from azobenzene and alkyl surfactants.
  • Cryo-transmission electron microscopy (TEM) and light-scattering for vesicle characterization.

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Published on: March 22, 2018

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Last Updated: Jun 23, 2026

Predicting Gene Silencing Through the Spatiotemporal Control of siRNA Release from Photo-responsive Polymeric Nanocarriers
11:53

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Published on: July 21, 2017

An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions
07:59

An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions

Published on: March 22, 2018

  • Fluorescent probe measurements to determine vesicle formation conditions.
  • Endocytosis studies in murine fibroblast NIH 3T3 cells.
  • DNA compaction and re-expansion studies using single-molecule fluorescence imaging.
  • Transfection efficiency assays with eGFP DNA.

    • Main Results:

    • Photoresponsive catanionic vesicles (50-200 nm) form spontaneously.
    • Visible light promotes vesicle formation at lower azobenzene surfactant concentrations (10 µM) compared to UV light (50-60 µM).
    • Vesicles facilitate DNA-vesicle complex uptake via endocytosis.
    • UV illumination triggers vesicle rupture and DNA release inside cells, enhancing gene expression.
    • Photoresponsive vesicles show higher cellular uptake and transfection efficiency than non-responsive controls.
    • DNA compaction and re-expansion occur rapidly upon vesicle formation and disruption.

    Conclusions:

    • Photoresponsive catanionic vesicles are effective gene delivery vectors.
    • Phototriggered release enhances DNA accessibility to the nucleus, improving transfection.
    • Enhanced cellular uptake is attributed to pi-pi stacking interactions increasing bilayer rigidity.
    • Vector unpacking and DNA release are critical steps in gene delivery efficiency.