[Screening method of pesticides in meat using cleanup with GPC and mini-column]

A multiresidue screening method using GC/MS and LC/MS was investigated for analysis of 202 pesticides (including metabolites) in meat. Many target pesticides with a wide range of polarity were successfully extracted with ethyl acetate-cyclohexane (1 : 1) from meat samples rich in lipid components. Matrix components such as cholesterol, monoglycerides and fatty acids were effectively eliminated by means of GPC and 2 kinds of mini-columns (PSA and Silica). These procedures enabled analysis in the SCAN mode by GC/MS and LC/MS without any matrix interference. Among the pesticides tested, 17 had low recoveries (<50%), but 185 pesticides showed acceptable recoveries of 50-140% when spiked at 0.1 microg/g into muscle of beef, chicken and pork. The limits of quantitation were 0.01 microg/g, except for linuron, triforine and isoxaflutole (<0.02 microg/g). This proposed method is expected to be useful for screening analysis of residual pesticides in meat.