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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

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Related Experiment Video

Updated: Jun 23, 2026

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)
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Ultrahigh resolution optical coherence tomography using a superluminescent light source.

Andrew Kowalevicz, Tony Ko, Ingmar Hartl

    Optics Express
    |May 14, 2009
    PubMed
    Summary
    This summary is machine-generated.

    A new superluminescent titanium-doped sapphire (Ti:Al2O3) crystal light source enables ultrahigh resolution optical coherence tomography (OCT). This robust alternative to femtosecond lasers achieves 1.7 microm resolution in tissue.

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    Area of Science:

    • Photonics and Biomedical Imaging

    Background:

    • Optical Coherence Tomography (OCT) requires high-bandwidth light sources for ultrahigh resolution imaging.
    • Conventional light sources like femtosecond lasers can be complex and costly.

    Purpose of the Study:

    • To demonstrate a superluminescent Ti:Al2O3 crystal as a robust and simple light source for ultrahigh resolution OCT.
    • To evaluate the performance of this Ti:Al2O3 light source in OCT imaging.

    Main Methods:

    • Utilized a thin Ti:Al2O3 crystal pumped by a frequency-doubled, diode-pumped laser.
    • Achieved fiber-coupled output powers of approximately 40 microW with a 138 nm bandwidth.
    • Integrated the Ti:Al2O3 light source into an OCT system.

    Main Results:

    • Demonstrated ultrahigh resolution OCT imaging with 2.2 microm axial resolution in air and 1.7 microm in tissue.
    • Achieved high sensitivity of >86 dB.
    • The Ti:Al2O3 crystal provided a stable, single spatial mode output.

    Conclusions:

    • The superluminescent Ti:Al2O3 crystal is a viable and effective light source for ultrahigh resolution OCT.
    • This approach offers a simpler and more robust alternative to femtosecond lasers for advanced OCT applications.