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Related Concept Videos

Computed Tomography01:10

Computed Tomography

Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
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Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
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Imaging Studies III: Computed Tomography

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Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

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Endoscopic Procedures III: Video Capsule Endoscopy01:28

Endoscopic Procedures III: Video Capsule Endoscopy

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Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

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Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography
08:50

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography

Published on: February 9, 2019

Video-rate three-dimensional optical coherence tomography.

Markus Laubscher, Mathieu Ducros, Boris Karamata

    Optics Express
    |May 14, 2009
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces fast three-dimensional optical coherence tomography (3D OCT) imaging at video rates. This breakthrough enables real-time 3D visualization of dynamic processes, overcoming previous time-consuming limitations.

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    Doppler Optical Coherence Tomography of Retinal Circulation
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    Published on: September 18, 2012

    Area of Science:

    • Biomedical Optics
    • Medical Imaging Technology
    • High-Speed Imaging

    Background:

    • Current optical coherence tomography (OCT) systems typically generate 2D images, requiring time-consuming sequential acquisition for 3D reconstructions.
    • Limitations in speed hinder the observation of rapid dynamic processes in biological tissues and materials.

    Purpose of the Study:

    • To demonstrate three-dimensional optical coherence tomography (3D OCT) at video rate for high-speed volumetric imaging.
    • To overcome the temporal limitations of conventional 3D OCT techniques.

    Main Methods:

    • Employed a 58x58 smart-pixel detector array for rapid data acquisition.
    • Achieved a frame rate of 25 Hz for 3D imaging, capturing volumes of 210x210x80 m³ (58x58x58 voxels).
    • Utilized a system with 3 µm longitudinal and 9 µm transverse resolutions and 76 dB sensitivity.

    Main Results:

    • Successfully demonstrated 3D OCT imaging at video rates (25 Hz).
    • Captured dynamic events, such as rapid thermal damage in a hair strand, in real-time 3D.
    • Achieved high spatial resolution (3 µm longitudinal, 9 µm transverse) at high speed.

    Conclusions:

    • Video rate 3D OCT represents a significant advancement in volumetric imaging speed.
    • This technology enables the real-time visualization and analysis of fast dynamic phenomena.
    • Opens new possibilities for in-situ studies of transient processes in various scientific fields.