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Published on: May 17, 2024
Nitric oxide inhibits the replication cycle of porcine parvovirus in vitro
Zhan-Yong Wei1, Xue-Bin Wang, Xiao-Dong Ning
1The College of Animal Science and Veterinary Medicine, Henan Agricultural University, Wenhua Road 95#, 450002, Zhengzhou, Henan, People's Republic of China. weizhanyong@hotmail.com
Abstract:
This study investigated the inhibitory effect and mechanism of nitric oxide (NO) on porcine parvovirus (PPV) replication in PK-15 cells. The results showed that two NO-generating compounds, S-nitroso-L-acetylpenicillamine (SNAP) and L-arginine (LA), at a noncytotoxic concentration could reduce PPV replication in a dose-dependent manner and that this anti-PPV effect could be reversed by the NO synthase (NOS) inhibitor N-nitro-L-arginine methyl ester (L-NAME). By assaying the steps of the PPV life cycle, we also show that NO inhibits viral DNA and protein synthesis. This experiment provides a frame of reference for the study of the anti-viral mechanism of NO.
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