Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Genetic analysis in vibrio.

M Silverman, R Showalter, L McCarter

    Methods in Enzymology
    |January 1, 1991
    PubMed
    Summary
    This summary is machine-generated.

    New genetic tools now enable comprehensive analysis of diverse Vibrio bacteria. These powerful methods combine recombinant DNA, transposon mutagenesis, and gene transfer for advanced research on undomesticated Vibrio species.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Perception of exercise induced asthma by children and their parents.

    Archives of disease in childhood·2003
    Same author

    Lung function and low birth weight.

    Archives of disease in childhood·2003
    Same author

    An adult model of exclusive viral wheeze: inflammation in the upper and lower respiratory tracts.

    Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2003
    Same author

    The economic impact of preschool asthma and wheeze.

    The European respiratory journal·2003
    Same author

    Screening for problem drinking in older people referred to a mental health service: a comparison of CAGE and AUDIT.

    Aging & mental health·2003
    Same author

    Efficacy of a targeted, oral penicillin-based yaws control program among children living in rural South America.

    Clinical infectious diseases : an official publication of the Infectious Diseases Society of America·2003
    Same journal

    Clinical Europium fluorescent based lectin assays for mucin O-glycomics.

    Methods in enzymology·2026
    Same journal

    A dual-color FRET assay for detection and quantitative analysis of O-glycopeptidases.

    Methods in enzymology·2026
    Same journal

    Evolutionary genetic approaches to analyze mucins.

    Methods in enzymology·2026
    Same journal

    Ex vivo imaging and enzymatic analysis of intestinal mucus.

    Methods in enzymology·2026
    Same journal

    Glyco-TRAPP: A real-time glycocalyx permeability assay for assessing transmembrane mucin barrier function in live and fixed tissues.

    Methods in enzymology·2026
    Same journal

    Quantitative imaging approaches to capture structural and functional dynamics of colonic mucus in health and disease in situ.

    Methods in enzymology·2026
    See all related articles

    Area of Science:

    • Microbiology
    • Genetics
    • Molecular Biology

    Background:

    • Bacteria of the genus Vibrio exhibit significant diversity.
    • Previous genetic analysis methods were often species-specific and fragmented.

    Purpose of the Study:

    • To present powerful and general strategies for the genetic analysis of Vibrio species.
    • To highlight the synergy of combining different molecular techniques.

    Main Methods:

    • Utilized recombinant DNA technology.
    • Employed innovations in transposon mutagenesis.
    • Applied transductive and conjugative gene transfer techniques.

    Main Results:

    • Developed powerful and general strategies for Vibrio genetic analysis.

    Related Experiment Videos

  • Demonstrated the synergy of combining recombinant DNA, transposon, and gene transfer methods.
  • Constructed transposons for gene fusions and broad host range plasmids for gene delivery and chromosome mobilization.

    • Conclusions:

    • Advanced genetic analysis is now feasible for numerous undomesticated Vibrio species.
    • The combined methodologies offer a powerful toolkit for Vibrio research.
    • Future studies can explore the vast, undomesticated Vibrio diversity with these new tools.