Related Experiment Video
Updated: Jun 23, 2026
Multimodal Approach to Assess Bone Regeneration and Scaffold Performance
Published on: February 13, 2026
Tracking of cellular uptake of hydrophilic CdSe/ZnS quantum dots/hydroxyapatite composites nanoparticles in MC3T3-E1
Ming-Fa Hsieh1, Jimmy Kuan-Jung Li, Cheng-An J Lin
1Department of Biomedical Engineering, R&D Center for Membrane Technology and Center for Nano Bioengineering, Chung Yuan Christian University, Chung-Li 32023, Taiwan, Republic of China.
Abstract:
We report the fluorescent labeling of osteoblast cells using the biocompatible hydroxyapatite (HA) grown with nucleating seed of hydrophilic CdSe/ZnS quantum dots (QDs) allowing the real-time observation of cell under confocal microscope. We found that the MC3T3-E1 osteoblast cells can engulf HA with surface-tailored QDs showing fluorescent spots in the cytoplasm, while HA and QDs nanoparticles were not engulfed. It is interesting to see that the fluorescence was only displayed in the cytoplasm of MC3T3-E1 osteoblast cells. It can be envisioned that the nano-sized hydroxyapatite bearing fluorescent QD can only be internalized in the cytoplasm. Therefore, it is worth utilizing these composite particles to observe cellular physiology with minimal toxicity to the osteoblast cells.
More Related Videos
07:31In Vivo Tracking of Human Adipose-derived Mesenchymal Stem Cells in a Rat Knee Osteoarthritis Model with Fluorescent Lipophilic Membrane Dye
Published on: October 8, 2017
09:35Distinctive Capillary Action by Micro-channels in Bone-like Templates can Enhance Recruitment of Cells for Restoration of Large Bony Defect
Published on: September 11, 2015