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iChip

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The cultivation of environmental microorganisms has long been hindered by the inability to replicate complex native conditions in vitro. The isolation chip (iChip) addresses this limitation by facilitating the growth of previously uncultivable microorganisms through in situ incubation. Designed for high-throughput microbial cultivation, the iChip comprises hundreds of microchambers, each capable of housing a single microbial cell. These microchambers are loaded with a mixture of molten agar and...

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Updated: Jun 23, 2026

Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals

Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals

Published on: February 14, 2019

Rewritable glycochips.

L G Harris¹, W C E Schofield, K J Doores

¹Department of Chemistry, Science Laboratories, Durham University, Durham DH1 3LE, UK.

Journal of the American Chemical Society

|May 15, 2009

Summary

This summary is machine-generated.

We developed a new method for carbohydrate microarraying to screen proteins. This technique overcomes nonspecific protein binding, enabling precise carbohydrate-protein interaction analysis.

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Area of Science:

Carbohydrate chemistry
Protein-carbohydrate interactions
Surface science

Background:

Carbohydrate microarrays are essential tools for studying protein-carbohydrate interactions.
Nonspecific protein binding is a common challenge in microarray assays, hindering accurate data interpretation.
Existing immobilization methods often struggle with background noise and limited spatial resolution.

Purpose of the Study:

To present a novel carbohydrate microarraying technique for efficient protein screening.
To address and overcome the issue of nonspecific protein binding in carbohydrate-based assays.
To enhance the spatial localization and specificity of carbohydrate-protein interactions.

Main Methods:

Carbohydrate immobilization onto functional nanolayers using disulfide bridge or Schiff base imine chemistries.
Development of a protein-resistant overlayer to prevent nonspecific binding.
Plasmachemical deposition of functional nanolayers for surface modification.

Main Results:

Successful microarraying of carbohydrates with high spatial localization.
Significant reduction in nonspecific protein background binding.
Demonstration of specific carbohydrate-protein interactions through the developed method.

Conclusions:

The described carbohydrate microarraying method effectively minimizes nonspecific binding.
This approach provides a robust platform for specific protein screening against carbohydrate arrays.
The technique holds promise for advancing glycomics and drug discovery research.