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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

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Hypoxia Alters miRNAs Levels Involved in Non-Mendelian Inheritance of Autism Spectrum Disorder in Mice
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MicroRNA-127 modulates fetal lung development.

Manoj Bhaskaran1, Yang Wang, Honghao Zhang

  • 1Lundberg-Kienlen Lung Biology and Toxicology Laboratory, Department of Physiological Sciences, Oklahoma State University, Stillwater, Oklahoma 74078, USA.

Physiological Genomics
|May 15, 2009
PubMed
Summary

MicroRNAs regulate gene expression. This study found microRNA-127 (miR-127) plays a key role in fetal lung development, impacting bud formation and size.

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Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNAs (miRNAs) are crucial regulators of gene expression in multicellular organisms.
  • Understanding miRNA roles in fetal lung development is essential for addressing congenital lung diseases.

Purpose of the Study:

  • To investigate the expression patterns of miRNAs during fetal lung development.
  • To identify specific miRNAs involved in regulating lung morphogenesis.
  • To elucidate the function of miRNA-127 (miR-127) in this process.

Main Methods:

  • miRNA microarray profiling to identify differentially expressed miRNAs.
  • Quantitative real-time PCR for validation.
  • In situ hybridization to determine cellular localization of miR-127.
  • Fetal lung organ culture to assess the functional impact of miR-127 overexpression.

Main Results:

  • 21 miRNAs exhibited significant expression changes during fetal lung development.
  • miRNAs were categorized into four expression clusters.
  • miR-127 expression increased in late-stage development and shifted from mesenchymal to epithelial cells.
  • Overexpression of miR-127 disrupted lung bud development, affecting count and size.

Conclusions:

  • miR-127 is dynamically regulated during fetal lung development.
  • miR-127 is implicated in the regulation of lung branching morphogenesis.
  • These findings highlight miR-127 as a potential target for therapeutic interventions in lung development disorders.