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1H NMR metabolomics study of age profiling in children

Haiwei Gu1, Zhengzheng Pan, Bowei Xi

  • 1Department of Physics, Purdue University, West Lafayette, IN 47907, USA.

NMR in Biomedicine
|May 15, 2009
PubMed

Insights

Urine metabolic profiling reveals age-related changes in children under 12. Key metabolites like creatinine, glycine, and citrate shift with age, offering insights into biological aging and metabolomics applications.

Area of Science:

  • Metabolomics
  • Biochemistry
  • Pediatric Research

Background:

  • Urinary metabolic profiling offers personalized endogenous metabolite markers.
  • These markers correlate with factors including age, gender, disease, diet, and medication.
  • Understanding individual factor contributions, like age, is crucial for accurate metabolomic interpretation.

Purpose of the Study:

  • To analyze age-related metabolic changes in children aged 12 and below.
  • To identify specific urinary metabolites that change with age in this demographic.
  • To explore the potential of urinary metabolomics in assessing biological age in children.

Main Methods:

  • (1)H NMR spectroscopy was used for urine metabolic profiling.
  • Unsupervised principal component analysis (PCA) was employed to observe age-dependent clustering.
  • Partial least squares with orthogonal signal correction (PLS-OSC) regression was used to identify age-related metabolic profiles.

Main Results:

  • Distinct age-dependent clustering of urinary metabolite profiles was observed via PCA.
  • PLS-OSC identified an age-related metabolic profile.
  • Creatinine increased with age, while metabolites including creatine, glycine, betaine/TMAO, citrate, succinate, and acetone decreased.

Conclusions:

  • Urinary metabolomics can reveal significant age-related shifts in children.
  • Specific metabolites show a clear correlation with chronological age in young individuals.
  • These findings may aid in assessing biological age and understanding confounding factors in pediatric metabolomics.

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