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Related Experiment Video

Updated: Jun 23, 2026

Deacetylation Assays to Unravel the Interplay between Sirtuins (SIRT2) and Specific Protein-substrates

Deacetylation Assays to Unravel the Interplay between Sirtuins (SIRT2) and Specific Protein-substrates

Published on: February 27, 2016

Sirtuin inhibitors.

Francisco J Alcaín¹, José M Villalba

¹Universidad de Córdoba, Departamento de Biología Celular, Fisiología e Inmunología, Facultad de Ciencias, Campus Rabanales, Edificio Severo Ochoa, Córdoba, Spain.

Expert Opinion on Therapeutic Patents

|May 16, 2009

Summary

This summary is machine-generated.

Sirtuin inhibitors offer potential therapeutic benefits for diseases like cancer and Parkinson's. By modulating sirtuin activity, these small molecules may increase lifespan and combat neurodegeneration.

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Last Updated: Jun 23, 2026

Deacetylation Assays to Unravel the Interplay between Sirtuins (SIRT2) and Specific Protein-substrates

Deacetylation Assays to Unravel the Interplay between Sirtuins (SIRT2) and Specific Protein-substrates

Published on: February 27, 2016

Area of Science:

Biochemistry
Molecular Biology
Pharmacology

Background:

The sirtuin (silent information regulator) family comprises seven NAD(+)-dependent deacetylase enzymes (SIRT1-7) with varied subcellular localization.
Sirtuin expression is generally upregulated by caloric restriction and oxidative stress.
SIRT1, a key mammalian sirtuin, deacetylates targets like the transcription factor p53, influencing lifespan and stress resistance.

Purpose of the Study:

To review the discovery and development of small molecules that modulate sirtuin activity.
To focus on sirtuin inhibitors and their therapeutic potential.

Main Methods:

Literature review of sirtuin inhibitors.
Analysis of reported mechanisms of action and therapeutic applications.

Main Results:

Specific SIRT1 inhibitors have been identified, potentially useful in cancer treatment by enhancing p53 activity to induce apoptosis.
One SIRT1 inhibitor demonstrates p53-independent apoptosis induction.
A selective SIRT2 inhibitor shows promise for treating Parkinson disease by reducing alpha-synuclein fibril formation.

Conclusions:

Sirtuin inhibitors represent a promising class of therapeutics for various human diseases.
SIRT1 inhibitors may be valuable in oncology and SIRT2 inhibitors in neurodegenerative disorders.