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Updated: Jun 23, 2026
Cryopreservation of Cortical Tissue Blocks for the Generation of Highly Enriched Neuronal Cultures
Published on: November 11, 2010
Cryopreservation of early postmitotic neuronal cells in culture
1Dublin City University, School of Biotechnology, Glasnevin, Dublin 9, Ireland. philippe.taupin@dcu.ie
Background:
The application is in the field of embryonic stem cells (ESCs) and the cryopreservation of neuronal cells in culture.
Objective:
It aims at establishing conditions for the cryopreservation of cultured neuronal cells readily usable, upon thawing. That is cryopreserved neuronal cells with a high rate of survivability and with a potential to differentiate into mature neuronal cells rapidly, after thawing.
Methods:
Neuronal precursor cells and neuronal cells were generated in vitro, from cultures of human ESCs (hESCs). Neuronal cells were frozen and cryopreserved at a stage when they begin to express neuronal class III beta-tubulin.
Results/Conclusion:
The application claims that neuronal cells elicit a rate of survivability greater than 50% and neurites outgrowth within 10 to 14 hours, after thawing. The cryopreservation of post-mitotic neuronal or neural-like cells provides a source of nerve cells readily usable for research and therapy.
