Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Dorsal Ligament Complex: A Cadaveric, Histology, and Imaging Study.

The Journal of hand surgery·2021
Same author

Interstitial fluid velocity is decreased around cortical bone vascular pores and depends on osteocyte position in a rat model of disuse osteoporosis.

Biomechanics and modeling in mechanobiology·2021
Same author

Micro- and Ultrastructural Characterization of Age-Related Changes at the Anterior Cruciate Ligament-to-Bone Insertion.

ACS biomaterials science & engineering·2021
Same author

Carbonyl iron and iron dextran therapies cause adverse effects on bone health in juveniles with chronic kidney disease.

Kidney international·2020
Same author

Polymeric mesh and insulin-like growth factor 1 delivery enhance cell homing and graft-cartilage integration.

Annals of the New York Academy of Sciences·2019
Same author

Botox-induced muscle paralysis alters intracortical porosity and osteocyte lacunar density in skeletally mature rats.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society·2019
Same journal

Corrigendum to "The forgotten fat: A systematic review of mandibular adipose content in health and disease" [Bone 201 (2025) 117636].

Bone·2026
Same journal

Diagnosis and management of X-linked hypophosphatemia in dental practice: A scoping review.

Bone·2026
Same journal

Baseline β-CTX and BMI predict suitability for deferred zoledronic acid redosing beyond 12 months in postmenopausal Indian women with osteoporosis.

Bone·2026
Same journal

Bone density-based maturation of the midpalatal suture in children aged 8-15 years.

Bone·2026
Same journal

Disrupted phosphate metabolism and SIBLING/ASARM peptide accumulation underlie impaired bone mineralization in klotho-deficient (kl/kl) mice.

Bone·2026
Same journal

Linking genetic variants to bone microstructure: Histological signatures of osteogenesis imperfecta subtypes.

Bone·2026
See all related articles

Related Experiment Video

Updated: Jun 23, 2026

Analysis and Imaging of Osteocytes
10:19

Analysis and Imaging of Osteocytes

Published on: November 29, 2024

An effective histological staining process to visualize bone interstitial fluid space using confocal microscopy.

Cesare Ciani1, Stephen B Doty, Susannah P Fritton

  • 1Department of Biomedical Engineering, City College of New York, New York, NY 10031, USA.

Bone
|May 16, 2009
PubMed
Summary
This summary is machine-generated.

Researchers developed a new staining method to visualize bone

More Related Videos

Automated Quantification of Hematopoietic Cell – Stromal Cell Interactions in Histological Images of Undecalcified Bone
09:31

Automated Quantification of Hematopoietic Cell – Stromal Cell Interactions in Histological Images of Undecalcified Bone

Published on: April 8, 2015

Simple Establishment of a Vascularized Osteogenic Bone Marrow Niche Using Pre-Cast Poly(ethylene Glycol) (PEG) Hydrogels in an Imaging Microplate
10:32

Simple Establishment of a Vascularized Osteogenic Bone Marrow Niche Using Pre-Cast Poly(ethylene Glycol) (PEG) Hydrogels in an Imaging Microplate

Published on: May 19, 2023

Related Experiment Videos

Last Updated: Jun 23, 2026

Analysis and Imaging of Osteocytes
10:19

Analysis and Imaging of Osteocytes

Published on: November 29, 2024

Automated Quantification of Hematopoietic Cell – Stromal Cell Interactions in Histological Images of Undecalcified Bone
09:31

Automated Quantification of Hematopoietic Cell – Stromal Cell Interactions in Histological Images of Undecalcified Bone

Published on: April 8, 2015

Simple Establishment of a Vascularized Osteogenic Bone Marrow Niche Using Pre-Cast Poly(ethylene Glycol) (PEG) Hydrogels in an Imaging Microplate
10:32

Simple Establishment of a Vascularized Osteogenic Bone Marrow Niche Using Pre-Cast Poly(ethylene Glycol) (PEG) Hydrogels in an Imaging Microplate

Published on: May 19, 2023

Area of Science:

  • Biomaterials Science
  • Bone Biology
  • Microscopy

Background:

  • Bone possesses a dual porosity: vascular and lacunar-canalicular.
  • Both porosities are crucial for interstitial fluid flow, vital for bone maintenance.
  • Visualizing and quantifying the small lacunar-canalicular porosity has been challenging.

Purpose of the Study:

  • To introduce a novel, reproducible staining protocol for bone microstructure.
  • To enable high-resolution visualization and quantification of interstitial fluid space.

Main Methods:

  • Utilized fluorescein isothiocyanate (FITC) as a fluorescent probe.
  • Employed confocal microscopy for visualization.
  • Developed a new staining protocol for bone tissue.

Main Results:

  • The FITC staining protocol reliably visualizes bone microstructure and microporosities.
  • Reconstructed cross-sections effectively display vascular and lacunar-canalicular networks.
  • High-resolution quantification of interstitial fluid space is now achievable.

Conclusions:

  • The new FITC staining method offers a reproducible way to study bone porosity.
  • This technique enhances the analysis of interstitial fluid dynamics in bone.
  • Applicable to both cortical and cancellous bone for detailed microstructural analysis.