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Updated: Jun 23, 2026

Imaging and Quantifying Mitochondrial Morphology in C. elegans During Aging

Imaging and Quantifying Mitochondrial Morphology in C. elegans During Aging

Published on: January 17, 2025

C. elegans longevity pathways converge to decrease mitochondrial membrane potential.

Bernard D Lemire¹, Maciej Behrendt, Adrienne DeCorby

¹Department of Biochemistry, University of Alberta, Alberta, Canada. bernard.lemire@ualberta.ca

Mechanisms of Ageing and Development

|May 16, 2009

Summary

Lowering mitochondrial membrane potential (DeltaPsi(m)) is linked to longer lifespan in C. elegans. This suggests longevity pathways converge on mitochondria, supporting the

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Published on: October 16, 2015

Area of Science:

Mitochondrial biology
Aging research
Genetics

Background:

Energy production via oxidative phosphorylation creates a mitochondrial membrane potential (DeltaPsi(m)).
Mitochondrial function is increasingly recognized as a key factor in the aging process.

Purpose of the Study:

To investigate the relationship between mitochondrial membrane potential (DeltaPsi(m)) and lifespan in Caenorhabditis elegans.
To determine if longevity pathways influence mitochondrial energetics.

Main Methods:

Measuring DeltaPsi(m) in long-lived mutants and wild-type C. elegans.
Utilizing RNA interference (RNAi) to study gene effects on DeltaPsi(m) and lifespan.
Employing the uncoupler carbonylcyanide-3-chlorophenylhydrazone (CCCP) to assess its impact on lifespan.

Main Results:

Long-lived C. elegans mutants exhibit a lower DeltaPsi(m) compared to wild-type animals.
The reduced DeltaPsi(m) in daf-2 mutants is dependent on daf-16, linking insulin signaling to mitochondrial energetics.
RNAi targeting lifespan-extending genes also decreased DeltaPsi(m).
Chemical dissipation of DeltaPsi(m) using CCCP significantly extended lifespan.

Conclusions:

Longevity pathways converge on mitochondria, leading to a decreased DeltaPsi(m).
These findings support the 'uncoupling to survive' hypothesis, where dissipating DeltaPsi(m) extends lifespan.
Mitochondrial energetics represent a critical target for interventions aimed at promoting longevity.