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Related Experiment Video

Updated: Jun 23, 2026

Sub-acute Cerebral Microhemorrhages Induced by Lipopolysaccharide Injection in Rats
06:39

Sub-acute Cerebral Microhemorrhages Induced by Lipopolysaccharide Injection in Rats

Published on: October 17, 2018

General 4-week toxicity study with EMS in the rat.

Thomas Pfister1, Anne Eichinger-Chapelon

  • 1Preclinical Research, F. Hoffmann-La Roche Ltd., PRNBT, Bldg 073/102c, CH-4070 Basel, Switzerland. thomas.pfister.tp1@roche.com

Toxicology Letters
|May 16, 2009
PubMed
Summary

Ethyl methanesulfonate (EMS) exposure in rats caused dose-dependent decreases in body weight and affected the hematopoietic, thymolymphatic, and reproductive systems. The low dose showed minimal adverse effects, indicating dose-limiting toxicity.

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Area of Science:

  • Toxicology
  • Pharmacology
  • Genotoxicity

Background:

  • Ethyl methanesulfonate (EMS) is a chemical agent used in research and industry.
  • Understanding the subacute toxicity profile of EMS is crucial for risk assessment.

Purpose of the Study:

  • To evaluate the subacute toxicity of EMS in Wistar rats following oral administration.
  • To identify target organs and dose-limiting effects of EMS exposure.

Main Methods:

  • Rats were administered EMS daily via oral gavage at doses of 20, 60, and 180/120 mg/kg bw for 28 days.
  • Hematology, histopathology, organ weights, and hemoglobin adducts were analyzed.
  • A 14-day recovery period was included for some groups.

Main Results:

Related Experiment Videos

Last Updated: Jun 23, 2026

Sub-acute Cerebral Microhemorrhages Induced by Lipopolysaccharide Injection in Rats
06:39

Sub-acute Cerebral Microhemorrhages Induced by Lipopolysaccharide Injection in Rats

Published on: October 17, 2018

  • Dose-dependent reductions in food consumption and body weight were observed, particularly at higher doses.
  • Primary target organs included the hematopoietic, thymolymphatic, and sexual systems, with effects on cell proliferation.
  • Hemoglobin ethylvaline adducts showed a linear dose-response relationship.

Conclusions:

  • Decreased food consumption and body weight were the dose-limiting effects of EMS.
  • EMS exhibits systemic toxicity affecting cell proliferation in multiple organ systems.
  • The lowest dose tested (20 mg/kg bw) had minimal adverse effects, despite detectable adducts.