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Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
Small interfering RNAs (siRNA)02:30

Small interfering RNAs (siRNA)

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...

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DNA Vector-based RNA Interference to Study Gene Function in Cancer
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Published on: June 4, 2012

RNA interference as a gene knockdown technique.

Ge Shan1

  • 1Department of Molecular, Cellular, and Developmental Biology, Yale University, P. O. Box 208103, New Haven, CT 06520, USA. ge.shan@yale.edu

The International Journal of Biochemistry & Cell Biology
|May 16, 2009
PubMed
Summary
This summary is machine-generated.

RNA interference (RNAi) is a powerful gene silencing technology. This review covers RNAi pathway basics, experimental applications in cells and organisms, and its role in developing new therapeutics.

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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Genetics

Background:

  • RNA interference (RNAi) is a significant scientific breakthrough with broad applications.
  • The elucidation of the RNAi pathway has spurred its use as a biotechnology.
  • RNAi is crucial for both basic research and translational medicine.

Purpose of the Study:

  • To review the RNA interference/microRNA pathway.
  • To summarize the design and application of RNA interference experiments.
  • To highlight recent progress and challenges in RNAi technology.

Main Methods:

  • Review of existing literature on RNA interference.
  • Detailed summary of RNAi experimental design and applications.
  • Discussion of RNAi utilization in animal cells and model organisms.

Main Results:

  • Small interference RNA and short-hairpin RNA are key reagents for gene knockdown.
  • RNAi technology has advanced therapeutic development, though challenges remain.
  • Morpholino and external guide sequence are discussed as complementary knockdown techniques.

Conclusions:

  • RNA interference is an indispensable tool in modern biological and medical research.
  • RNAi technology offers significant advances in both basic science and translational applications.
  • Continued research addresses remaining hurdles in RNAi application and development.