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Related Experiment Video

Updated: Jun 23, 2026

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

An aptamer-based electrochemiluminescent biosensor for ATP detection.

Wu Yao¹, Lun Wang, Haiyan Wang

¹Anhui Key Laboratory of Chemo-Biosensing, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China.

Biosensors & Bioelectronics

|May 16, 2009

Summary

A novel aptamer-based biosensor detects adenosine triphosphate (ATP) with high sensitivity. This electrochemiluminescent system utilizes complementary DNA as probes for label-free small-molecule detection and regeneration.

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Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

Area of Science:

Biomedical Engineering
Analytical Chemistry
Biosensing Technology

Background:

Adenosine triphosphate (ATP) is a crucial biomarker for various biological processes.
Existing biosensors for ATP often face challenges in sensitivity, specificity, or regeneration.
Aptamer-based biosensors offer high specificity but require innovative probe designs for improved performance.

Purpose of the Study:

To develop a highly sensitive and specific aptamer-based electrochemiluminescent (ECL) biosensor for ATP detection.
To explore a novel sensing strategy using complementary DNA (cDNA) as the primary probe for ECL sensing.
To establish a label-free and readily regenerated platform for small-molecule detection.

Main Methods:

Assembly of an ECL biosensor involving hybridization of an ATP-binding aptamer with its cDNA.
Labeling of the resulting double-stranded DNA (ds-DNA) with a ruthenium complex.
Immobilization of the ds-DNA onto a gold electrode surface.
Detection of ATP based on the dissociation of the aptamer from ds-DNA, leading to increased ECL signal.

Main Results:

The biosensor demonstrated high sensitivity and specificity for ATP detection.
A linear relationship was observed between ECL intensity and the logarithm of ATP concentration from 0.05 nM to 10 nM.
A low detection limit of 0.02 nM for ATP was achieved.
The proposed system utilizes cDNA as the probe, differentiating it from other aptamer-based ECL biosensors.

Conclusions:

The developed aptamer-based ECL biosensor offers a promising approach for sensitive ATP quantification.
The innovative use of cDNA as the probe enables a label-free and easily regenerated sensing platform.
This strategy holds potential for the development of advanced aptamer-based biosensors for various small molecules.