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Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Chromatin Structure and RNA Splicing02:41

Chromatin Structure and RNA Splicing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...

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Related Experiment Video

Updated: Jun 23, 2026

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

Structural insights into RNA splicing.

Navtej Toor1, Kevin S Keating, Anna Marie Pyle

  • 1Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511, USA.

Current Opinion in Structural Biology
|May 16, 2009
PubMed
Summary
This summary is machine-generated.

This study compares the structures and catalytic mechanisms of self-splicing introns (group I and II) with the spliceosome. Findings may illuminate spliceosome mechanisms in nuclear pre-mRNA splicing.

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Last Updated: Jun 23, 2026

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
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Using the E1A Minigene Tool to Study mRNA Splicing Changes
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Published on: April 22, 2021

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Structural Biology

Background:

  • Intron splicing removes noncoding sequences from precursor RNAs.
  • Group I and II introns are self-splicing RNAs with known structures and mechanisms.
  • The spliceosome is a eukaryotic ribonucleoprotein machine for nuclear pre-mRNA splicing.

Purpose of the Study:

  • To compare the structures and catalytic mechanisms of self-splicing RNAs (group I and II introns).
  • To discuss potential implications of self-splicing intron mechanisms for spliceosomal function.

Main Methods:

  • Comparative structural analysis of group I and II introns.
  • Review of existing structural and mechanistic data for the spliceosome.
  • Functional and mechanistic comparison between self-splicing introns and the spliceosome.

Main Results:

  • Structural similarities and differences between self-splicing introns and spliceosome components.
  • Mechanistic parallels and divergences in RNA catalysis.
  • Insights into the evolution of RNA-based catalytic processes.

Conclusions:

  • Self-splicing introns provide models for understanding RNA catalysis.
  • Comparative analysis suggests potential evolutionary links and mechanistic principles shared between self-splicing RNAs and the spliceosome.
  • Further structural and mechanistic studies are needed to fully elucidate spliceosome function.