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Updated: Jun 23, 2026
Techniques to Induce and Quantify Cellular Senescence
Published on: May 1, 2017
[Experimental study on effect of total flavone of Ginkgo biloba on delaying cell senescence]
Xiao-ping Song1, Zhi-wu Chen, An-ning Fang
1Department of Biology, Anhui Medical College, Hefei 230061, China. sxp@ahyz.cn
Objective:
To investigate the mechanism of delaying the senescence of human diploid fibroblast (2BS) by total flavone of Ginkgo biloba (FG).
Methods:
The drug sera of FG was used to treat the 2BS. The population doublings of 2BS cells were observed, the mRNA expression of P16 gene was determined by fluorescence real-time quantitative PCR.
Results:
FG significantly extended the population doublings of 2BS cells, and decreased the expression of P16 mRNA.
Conclusion:
FG can delay the senescence of cells by inhibiting the P16 gene expression.
Insights
Total flavone of Ginkgo biloba (FG) delays cellular senescence by inhibiting P16 gene expression in human fibroblast cells. This research explores FG
Area of Science:
- Cellular senescence research
- Molecular biology
- Pharmacology
Background:
- Cellular senescence is a key factor in aging.
- Human diploid fibroblasts (2BS) are a model for studying senescence.
- Ginkgo biloba extracts have shown potential anti-aging properties.
Purpose of the Study:
- To investigate the mechanism by which total flavone of Ginkgo biloba (FG) delays senescence in 2BS cells.
- To determine the effect of FG on P16 gene expression.
Main Methods:
- Treatment of 2BS cells with FG.
- Observation of cell population doublings.
- Quantification of P16 mRNA expression using fluorescence real-time quantitative PCR.
Main Results:
- FG significantly extended the population doublings of 2BS cells.
- FG treatment led to a decrease in P16 mRNA expression.
Conclusions:
- Total flavone of Ginkgo biloba (FG) effectively delays cellular senescence.
- FG exerts its anti-senescence effect by inhibiting the expression of the P16 gene.