[Study on optimization of formulation and preparation process of sinomenine liposomes]
Yan Wang1, Zhenna Cong, Qingfei Liu
1Section of Chinese Pharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, China. gdpuwy@126.com
Objective:
To optimize the formulation and preparation process of sinomenine liposomes.
Method:
Method of aether injection and mixture uniform design were adopted to determine the formulation of sinomenine liposomes is the proportion of phospholipids, cholesterol and Vitamin E with the index of entrapment efficiency. And the single-factor test was used to study the preparation process of the liposomes, including the volume of buffer solution, the preparation temperature and the ultrasonic time.
Result:
The optimized formulation was that the ratio of sinomenine : phospholipids : cholesterol : vitamin E mass ratio was 8.92 : 60.35 : 28.81 : 1.91. The volume of buffer solution was 50 mL x g(-1) membrane, the preparation temperature was 50 degrees C, and the ultrasonic time was 20 min.
Conclusion:
Satisfactory shape and entrapment efficiency of the liposomes can be obtained by the optimized formulation and preparation process.
