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[RP-HPLC determination of tiliroside in Daphne genkwa]
Lili Song1, Juan Lu, Peifang Yan
1College of Chemistry of JiLin University, Changchun 130012, China.
Objective:
To determine the contents of tiliroside [kaempferol 3-O-beta-D-(6 " -p-coumaroyl) glucopyranoside] in the Daphne genkwa.
Method:
The separation was performed on ZORBAX XDB C18 column (4.6 mm x 250 mm, 5 microm), using acetonitrile and water/acetic acid mixture (1,000/1) (gradient elution: 0-18 min, 26 : 74; 18-33 min, 80 : 20) as mobile phase with the rate of 1.0 mL x min(-1) at 25 degrees C, the wavelength for measurement was 310 nm.
Result:
The linearity was in the range of 0.1-5.0 microg (n=6), and the correlation coefficient was 0.9999. The average recovery for compound tiliroside was 101.50% (RSD 0.90%) (n=6).
Conclusion:
This method is accurate, reliable and reproducible to determine tiliroside in the D. genkwa.
