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[Determination of oleuropein in stem of Syringa oblata from different districts by HPLC]
Xiaohui Wei1, Shuxia Zhang, Weifeng Zhai
1Shanghai R&D Center for Standardization of Traditional Chinese Medicine, Shanghai 201203, China. xhweixh@163.com
Objective:
To develop an HPLC method for the determination of oleuropein in Syringa oblata.
Method:
An Aigilent ZORBAX SB- C18 column (4.6 mm x 250 mm, 5 microm) was used. The mobile phase was acetonitrile-water (21 : 79). The flow rate was 1.0 mL x min(-1). The detection wavelenghth was set at 232 nm and the column temperature was 30 degrees C.
Result:
The linear range of oleuropein were from 0.011 62 g x L(-1) to 1.162 g x L(-1). The average recovery was 98.7% with RSD 2.5% (n=9).
Conclusion:
The method is reliable, accurate and specific. It can be used for quality control of the stem of Syringa oblata.
