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High-resolution Respirometry to Assess Mitochondrial Function in Permeabilized and Intact Cells
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Involvement of respiratory chain in the regulatory volume decrease process in turbot hepatocytes.

Audrey Fouchs1, Hélène Ollivier, Michael Theron

  • 1Laboratoire ORPHY, Universite Europeenne de Bretagne, Universite de Brest, 6 Avenue Le Gorgeu CS 93837, 29238 Brest-CEDEX 3, France.

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
|May 19, 2009
PubMed
Summary
This summary is machine-generated.

Turbot hepatocytes use mitochondrial respiration indirectly for regulatory volume decrease (RVD) under hypo-osmotic stress. ATP is synthesized and stored in vesicles for release, but exocytosis requires further investigation.

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Area of Science:

  • Cellular Biology
  • Fish Physiology
  • Mitochondrial Respiration

Background:

  • Regulatory volume decrease (RVD) is a key cellular response in turbot hepatocytes to hypo-osmotic stress.
  • Extracellular ATP (adenosine triphosphate) release is implicated in signaling during RVD, but its origin and the role of mitochondria are unknown.

Purpose of the Study:

  • To investigate the link between mitochondrial respiration and RVD in turbot hepatocytes.
  • To elucidate the role of ATP release and exocytosis in this process.

Main Methods:

  • Turbot hepatocytes were exposed to hypo-osmotic stress.
  • Mitochondrial respiration was inhibited using specific chemical inhibitors.
  • ATP release and cellular volume changes were monitored.

Main Results:

  • RVD was dependent on mitochondrial respiratory chain activity.
  • Mitochondrial respiration plays an indirect role, facilitating ATP synthesis and storage in intracellular vesicles.
  • ATP is released from vesicles to mediate RVD during hypo-osmotic stress.

Conclusions:

  • Mitochondrial respiration is essential for RVD in turbot hepatocytes, primarily through ATP synthesis and storage.
  • The release mechanism, potentially involving exocytosis, warrants further investigation.