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Updated: Jun 23, 2026

Chromatin Immunoprecipitation (ChIP) using Drosophila tissue
Published on: March 23, 2012
[Preconcentration and separation of DNA fragments based on microchip electrophoresis]
1Department of Chemistry & Chemical Engineering, Graduate School of Engineering, Hiroshima University, Higashi-Hiroshima 739-8527, Japan. chemxzq@hiroshima-u.ac.jp
Abstract:
An online preconcentration method, electrokinetic supercharging (EKS) was used for the enrichment of DNA fragments based on microchip electrophoresis (MCE). EKS is a process that combines electrokinetic injection (EKI) with transient isotachophoresis (tITP). The results demonstrated that the large volume of low concentration sample could be introduced, preconcentrated, and eventually separated on a single channel microchip with the whole length of 40.5 mm. The limit of detection (S/N = 3) of DNA fragments was around 0.07 mg/L, effectively improved 40-fold by EKS preconcentration with the normal UV detection at 260 nm. Some important parameters for enhancing preconcentration and qualitative analysis were examined.
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