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[Simultaneous determination of artificial sweeteners in beverage by ultra performance liquid chromatography]
Chao Ji1, Yanyan Sun, Xiuqin Li
1State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214036, China.
Abstract:
An ultra performance liquid chromatographic (UPLC) method for the simultaneous separation and determination of four artificial sweeteners (sodium saccharin, aspartame, acesulfame and neotame) in a single injection was developed. The separation was performed on an ACQUITY UPLC BEH C18 column with gradient program and detection at 220 nm. The good linearities between the concentrations of all analytes and peak area responses were achieved over the range from 0.5 to 20.0 mg/L. The average recoveries in samples were 80.5% - 95.2% with the relative standard deviations of 0.50% - 8.7%. The method has been successfully applied to the determination of the four sweeteners in drinks and powdered tabletop sweeteners.
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