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Updated: Jun 23, 2026

Hydrogen Production and Utilization in a Membrane Reactor
Published on: March 10, 2023
Hydrogen production by a hyperthermophilic membrane-bound hydrogenase in water-soluble nanolipoprotein particles
Sarah E Baker1, Robert C Hopkins, Craig D Blanchette
1Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, USA.
Abstract:
Hydrogenases constitute a promising class of enzymes for ex vivo hydrogen production. Implementation of such applications is currently hindered by oxygen sensitivity and, in the case of membrane-bound hydrogenases (MBHs), poor water solubility. Nanolipoprotein particles (NLPs) formed from apolipoproteins and phospholipids offer a novel means of incorporating MBHs into a well-defined water-soluble matrix that maintains the enzymatic activity and is amenable to incorporation into more complex architectures. We report the synthesis, hydrogen-evolving activity, and physical characterization of the first MBH-NLP assembly. This may ultimately lead to the development of biomimetic hydrogen-production devices.
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