Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mesenchymal Stem Cells01:19

Mesenchymal Stem Cells

4.5K
Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
4.5K
iPS Cell Differentiation01:22

iPS Cell Differentiation

2.2K
The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
2.2K
Forced Transdifferentiation01:28

Forced Transdifferentiation

1.5K
Transdifferentiation, also known as lineage reprogramming, was first discovered by Selman and Kafatos in 1974 in silkmoths. They observed that the moths’ cuticle-producing cells transformed into salt-producing cells. Many such cases of natural transdifferentiation occur in organisms. In humans, pancreatic alpha cells can become beta cells. In newts, the loss of the eye’s lens causes the pigmented epithelial cells to transdifferentiate into the lens cells.
Artificial...
1.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The NORAD-Pumilio regulatory axis in the evolution of inclusion body myositis.

Journal of neuropathology and experimental neurology·2026
Same author

Oxidative stress sensing by the translation elongation machinery promotes production of detoxifying selenoproteins.

bioRxiv : the preprint server for biology·2025
Same author

<i>Plagl1</i> and <i>Lrrc58</i> control mammalian body size by triggering target-directed microRNA degradation of miR-322 and miR-503.

Genes & development·2025
Same author

Structural basis for the dynamic regulation of mTORC1 by amino acids.

Nature·2025
Same author

From Protein Folding to Precision Therapy: Surfactant Protein A in the Spotlight.

American journal of respiratory cell and molecular biology·2025
Same author

Polyglycine-mediated aggregation of FAM98B disrupts tRNA processing in GGC repeat disorders.

Science (New York, N.Y.)·2025

Related Experiment Video

Updated: May 5, 2026

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

10.1K

Abate and switch: miR-145 in stem cell differentiation.

Raghu R Chivukula1, Joshua T Mendell

  • 1The McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

Cell
|May 20, 2009
PubMed
Summary

MicroRNAs regulate embryonic stem cell renewal. miR-145 promotes differentiation by inhibiting key pluripotency factors OCT4, SOX2, and KLF4, thus stopping self-renewal.

Area of Science:

  • Stem cell biology
  • Molecular biology
  • Epigenetics

Background:

  • Embryonic stem (ES) cells possess self-renewal and pluripotency.
  • MicroRNAs (miRNAs) are recognized as crucial regulators in these processes.

Purpose of the Study:

  • To investigate the role of miR-145 in regulating ES cell self-renewal and pluripotency.
  • To identify the specific targets of miR-145 in the context of pluripotency.

Main Methods:

  • Analysis of miRNA expression during ES cell differentiation.
  • Inhibition of miR-145 and assessment of its effects on pluripotency markers.
  • Reporter assays to confirm repression of target genes.

Main Results:

  • miR-145 expression is upregulated during ES cell differentiation.

More Related Videos

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes
10:48

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Published on: April 12, 2015

11.7K
Transient Treatment of Human Pluripotent Stem Cells with DMSO to Promote Differentiation
06:55

Transient Treatment of Human Pluripotent Stem Cells with DMSO to Promote Differentiation

Published on: July 17, 2019

14.1K

Related Experiment Videos

Last Updated: May 5, 2026

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

10.1K
Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes
10:48

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Published on: April 12, 2015

11.7K
Transient Treatment of Human Pluripotent Stem Cells with DMSO to Promote Differentiation
06:55

Transient Treatment of Human Pluripotent Stem Cells with DMSO to Promote Differentiation

Published on: July 17, 2019

14.1K
  • Overexpression of miR-145 promotes differentiation and reduces pluripotency.
  • miR-145 directly represses the core pluripotency factors OCT4, SOX2, and KLF4.

    • Conclusions:

    • miR-145 acts as a key facilitator of ES cell differentiation.
    • By targeting OCT4, SOX2, and KLF4, miR-145 silences the self-renewal program.
    • This finding provides insight into the molecular mechanisms governing stem cell fate decisions.